百科

2-硝基苯-beta-D-半乳糖苷

CAS号：369-07-3

英文名：2-Nitrophenyl-beta-D-galactopyranoside

分子式

C12H15NO8

熔点

186-193°C (dec.)

密度

1.599 g/cm3

分子量

301.249

沸点

572.4ºC at 760 mmHg

溶解度

信号词

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化合物简介

ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity by means of a colorimetric assay (at 420nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used as a measure of the enzymatic rate.

基本信息

中文名称

2-硝基苯-beta-D-半乳糖苷

英文名称

2-Nitrophenyl-beta-D-galactopyranoside

中文别名

邻硝基苯-β-D-吡喃半乳糖苷、2-硝基苯基-β-D-吡喃半乳糖苷(ONPG)、2-硝基苯基-β-D-吡喃半乳糖苷、邻硝基苯基-β-D-吡喃半乳糖苷、邻硝基苯-β-D-半乳糖苷(ONPG)、2-硝基苯基-b-D-半乳糖苷、2-硝基苯基β-D-吡喃半乳糖苷、2-氟代-2-去氧-D-葡萄糖

英文别名

2-Nitrophenyl β-D-galactopyranoside、2-Nitrophenyl β-D-Galactopyranoside [Substrate、2-Nitrophenyl β-D-galactopyranoside (ONPG)、2-Nitrophenyl beta-D-Galactopyranoside、2-Nitrophenyl-β-D-galactopyranoside、2-Nitrophenyl-β-D-Galactopyranoside

CAS号

369-07-3

分子式

C12H15NO8

分子量

301.249

精确质量

301.08

PSA

145.2

LOGP

-0.7033