化合物简介
5-Bromo-4-chloro-3-indolyl phosphate (BCIP, X-phosphate, XP) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity. It is, for example, used in immunoblotting, in situ hybridization, and immunohistochemistry, often in combination with nitro blue tetrazolium chloride (NBT). 5-bromo-4-chloro-3-indoxyl is oxidized by atmospheric oxygen to form the blue dye 5,5′-dibromo-4,4′-dichloro-indigo. It is also oxidized by nitroblue tetrazolium (NBT), which forms an insoluble dark blue diformazan precipitate after reduction. Alkaline phosphatase is commonly conjugated to secondary antibodies.
基本信息
中文名称
5-溴-4-氯-3-吲哚基磷酸盐
英文名称
5-bromo-4-chloro-3-indolyl phosphate
中文别名
英文别名
5-bromo-4-chloroindoxyl phosphate、5-Bromo-4-chloro-3-indolyl phosphate、Fragment compound,6、Indole der.、5-Bcip、BCIP、5-Bromo-4-chloro-3-indoxyl phosphate、(5-bromo-4-chloro-1H-indol-3-yl) dihydrogen phosphate
CAS号
38404-93-2
分子式
C8H6BrClNO4P
分子量
326.468
精确质量
324.891
PSA
92.36
LOGP
3.0553
物化性质
密度
2.083 g/cm3
沸点
580.2ºC at 760 mmHg
闪点
304.7ºC
安全信息
海关编码
2933990090